Journal: Developmental biology

Article Title: Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres

doi: 10.1016/j.ydbio.2019.01.010

Figure Lengend Snippet: Culture medium optimized for trunk crestospheres.

Article Snippet: Due to rapid degradation, retinoic acid and BMP-4 were re-added to the culture medium volume every 2–3 days, and the spheres were also gently dispersed by pipetting up and down ~ 10–20 times against the culture flask wall every 2–3 days. table ft1 table-wrap mode="anchored" t5 caption a7 Medium (NC) C Final DMEM (with P/S) Chicken embryo extract 7.5% B27 1 × IGF-I 20 ng/ml FGF 20 ng/ml Retinoic Acid 180 nM BMP-4 25 ng/ml DMEM Corning #10–013-CVR, with 4.5 g/L Glucose Chick Embryo Extract (CEE) MP Biomedicals #092850145 B27 Life Technologies #12587–010 – 50 × Human recombinant IGF-I Sigma Aldrich #SRP3069 – in ddH2O + 5% Trehalose in PBS (1:1) Human recombinant bFGF Peprotech – in Tris 5 mM pH7.6 Retinoic acid Sigma Aldrich #R2625–10 mM in DMSO BMP-4 Peprotech #120–05 – in citric acid (pH3.0): BS with 2 mg/ml BSA (1:1) Open in a separate window Culture medium optimized for trunk crestospheres.

Techniques: Recombinant